- S100B is involved in the regulation of energy metabolism in brain cells.
- S100B is produced primarily by astrocytes and exerts autocrine and paracrine effects on glia, neurons, and microglia.
- Antibodies raised to S100B have been shown to label primarily astrocytes in human brain (Lyck et al. 2008).
- Spatiotemporal expression during brain development, particularly in proliferating and mature astrocytes (Marshak 1990; Tiu et al. 2000).
|Promoter Constructs||ESC Lines||Mice|
|pEMS1382||mEMS2742, mEMS2743, mEMS2744, mEMS2749||mice generated using ESC mEMS2742|
|pEMS1384||mEMS1603, mEMS1604, mEMS1610, and mEMS1627||mice generated using ESC mESM1627|
|pEMS1385||mEMS2748, mEMS2752, mEMS2759, mEMS2761||mice generated using ESC mESM2748|
pEMS1384 strain shows specific staining in the cerebellum, labeling Bergman glial cells. A) Brightfield micrograph from the cerebellum. Anti-GFP staining (brown DAB reaction product) reveals radial processes of Bergman glia as well as in the cell bodies (small arrowheads) and pial endfeet (large arrowheads) B) Confocal laser scanning micrograph of a thin optical section in the cerebellum. Double label in the cerebellum of GFP fluorescence (green) and anti-GFAP immunoflourescence (red) clearly shows similar chimeric staining in the Bergman glia. GFP fluorescence highlights Bergman glial cell bodies (small arrowheads) and decorates processes (large arrowheads) that follow the GFAP (red) positive GFAP-positive radial processes. PCL, Purkinje cell layer; ML Molecular Layer, pia, apical pial surface.